CV

1. Investigation of structure of a protein when it is still associated with the ribosome (nascent chain) using Cellular Retinoic acid binding protein I, CRABP I, as a model system, using FRET based methodology.
2. Development of a novel His tag binding fluorophore.
3. Biophysical characterization of circular permutant of CRABP I.
4. Characterization of a split tetra Cys FlAsH binding motif using CRABP I as a model system in order to use it as a specific fluorescence probe to investigate long range interactions in protein.