University of Massachusetts
Department of Chemistry
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 © 2007
University of Massachusetts Amherst



Caspase Papers
  Judy Ventura, Scott J. Eron, Daniella C. Gonzalez-Toro, Kishore Raghupathi, Feng Wang, Jeanne A Hardy*, and S. Thayumanavan* (2015) Reactive Self-Assembly of Polymers and Proteins to Reversibly Silence a Killer Protein. Biomacromolecues. DOI: 10.1021/acs.biomac.5b00779  
  Mout R, Zhao Y, Yeh YC, Tang R, Jeong Y, Duncan B, Hardy JA, Rotello VM. (2015) Co-Delivery of Protein and Small Molecule Therapeutics Using Nanoparticle-Stabilized Nanocapsules. Bioconjug Chem. 26 (5), 950–954.  
  Dagbay. K.B., Eron. S.J., Serrano, B.P., Zhao, Y., Velázquez-Delgado, E.M., Lin, D. and Hardy, J.A. (2014). A multi-pronged approach for compiling a global map of allosteric regulation in the apoptotic caspases. Methods in Enzymology, UK: Academic Press, 544: 215-249.  
  Tang R, Kim CS, Solfiell DJ, Rana S, Mout R, Velázquez-Delgado EM, Chompoosor A, Jeong Y, Yan B, ZhuZJ, Kim C, Hardy JA, Rotello VM. (2013) Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules. ACS Nano. 7(8):6667-73.  

Velazquez-Delgado, E. M. and Hardy, J. A., 2012. “Zinc-Mediated Allosteric Inhibition of Caspase-6.”Journal of Biological Chemistry. 287(43), 36000.

  Huber, K.L. and Hardy J.A., 2012 “Mechanism of zinc-mediated inhibition of caspase-9.” Protein Science. 21,1056-1065.. Article featured as cover illustration. pdf
  Huber, K.L., Ghosh, S. and Hardy J.A., 2012. “Inhibition of caspase-9 by stabilized peptides targeting the dimerization interface.”Peptide Science. 98(5), 451–465. pdf
  Velazquez-Delgado, E. M. and Hardy J.A., 2012. “Phosphorylation regulates assembly of the caspase-6 substrate-binding groove.” Structure. 20, 742–751.

pdf; preview; press release

  Vaidya S., Hardy J.A., Caspase-6 Latent State Stability Relies on Helical Propensity.
Biochemistry 2011, 50(16):3282-7.
  Vaidya S., Velázquez-Delgado E.M., Abbruzzese G., Hardy J.A. Substrate-Induced Conformational Changes Occur in All Cleaved Forms of Caspase-6. Journal of Molecular Biology 2011, 406(1):75-91. pdf
  Witkowski, W.A., Hardy, J.A. "L2 loop is critical for caspase-7 active site formation" Protein Science, 2009, 18, 1459-1468. Article featured as cover illustration.


Witold's movie of conformational changes in the capsase mutant I213A.
  Hardy, J. A. and Wells, J.  “Dissecting an Allosteric Switch in Caspase-7 using Chemical and Mutational Probes.” Journal Biological Chemistry 2009, 284(38):26063-9. pdf
  Hardy, J. A., Lam, J., Nguyen, J. T., O’Brien, T. and Wells, J. “Discovery of an allosteric site in caspases.” Proc. Natl. Acad. Sci. U S A. 2004, 101(34), 12461-6. pdf
Protein Design Papers  
  Maureen E. Hill, Derek J. MacPherson, Peng Wu, Olivier Julien, James A. Wells, Jeanne A. Hardy 2015 “Reprogramming Caspase-7 specificity to Caspase-6 by regio-specific mutations and selection” Under Revision ACS Chemical Biology




Wu, P., Nicholls, S., and Hardy, J.A. 2013 “A tunable, modular approach to fluorescent protease-activated reporters.”Biophysical Journal. 104(7):1605-14.


Nicholls, S. and Hardy, J.A. 2013 “Structural Basis of Fluorescence Quenching in Caspase Activatable-GFP.” Protein Science. 22(3), 247. Article featured as cover illustration.

  López, G.E, Colón-Díaz, I., Cruz, A., Ghosh,S., Nicholls, S.B., Viswanathan, U., Hardy, J.A. and Auerbach, S.M. 2012 “Modeling non-aqueous proton wires tethered to helical peptides: Biased proton transfer driven by helical dipoles.” The Journal of Physical Chemistry A. 116(4):1283-8. pdf

Nicholls, S., Chu, J., Abbruzzese, G. Tremblay, K.D. and Hardy, J.A. “Mechanism of a dark-to- bright reporter of caspase activity.”Journal of Biological Chemistry, 286 (28), 24977-24986.

pdf; press release

CA-GFP Movie


Witkowski, W. and Hardy, J.A. “A designed redox-controlled caspase”Protein Science, 20, 1421-1431. Article featured as cover illustration.

  Huber, K.L., Olson, K.D. and Hardy, J.A. "Robust production of a peptide library using methodological synchronization" Protein Expression and Purification. 2009, 67,139-147. pdf
  Hardy, J. A. and Nelson, H. C. M. “Proline in an alpha-helical kink is required for folding kinetics but not for kinked structure, function or stability of heat shock transcription factor” Protein Science, 2000, 9(11), 2128-2141. pdf
  Hardy, J. A., Walsh, S. T. R., and Nelson, H. C. M. “Role of an alpha-Helical Bulge in the Yeast Heat Shock Transcription Factor” J. Mol. Biol., 2000, 295(3), 393-409. pdf
Allosteric Papers  
  Hardy, J. A. and Wells, J. “Searching for Allosteric Sites in Enzymes.” Curr. Opin. Str. Biol. 2004, 14(6), 706-715. pdf
  Hardy, J.A. "Discovery and exploitation of allosteric sites for control of protein function." Nanomedicine: Nanotechnology, Biology and Medicine 2006, 2(4), 291.  

Hardy, J. A. “A Link Means a Lot: Disulfide Tethering in Structure-Based Drug Design” in Computational Approaches to Structure Based Drug Design, publishers Royal Society of Chemistry, 2007, 318-347.

  Smith Family Young Investigator Award from The Medical Foundation 2005-2007  
  Beckman Young Investigator Award from the Arnold and Mabel Beckman Foundation 2006-2010
  American Cancer Society Institutional Research Grant 2006-2007  
  NSF Chemical Bonding Center "Fueling the Future" 2007-2010  
  NIH R01 Grant 2008-2018  
  NSM Excellence Initiative Grant 2008  
  Cottrell Scolar Award from Research Corporation 2008-2012  
  Center of Excellence in Apoptosis Grant 2009-2012  
  NIH R03 Grant 2012-2014  
  Armstrong Grant 2013-2015  



Lab Updates

Bay Serrano wins best talk prize at ResearchFest. Kevin Dagbay and Scott Eron win poster prizes!

Scott Eron's paper in collaboration with the Thai lab was accepted in Biomacromolecules .7-24-15
NIH awards supplement for new Hardy Lab plate reader.










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