HIV RT-1: General Features The structure at right is the unliganded human immunodeficiency virus, reverse transcriptase 1 (HIV RT-1). The RT of HIV-1 has two enzymatic activities: a DNA polymerase that can copy either DNA or RNA templates and an RNAse H. HIV RT-1 is a heterodimer composed of domains p66 (larger) and p51 (smaller). The enzyme has an approximate total mass of 117 kDa.
p66 p51 Both subunits derive from the same polyprotein precursor and are later processed by the viral protease to yield the final active enzyme. p66 is composed of 560 amino acids; p51 has 440 amino acids. Due to similar origins, each corresponding amino acid sequence in each subunit shows a great deal of homology. HIV RT-1 Subdomains: fingers palm thumb Binding Cleft The binding cleft of the dsDNA template-primer is composed of the finger, palm and the thumb subdomains. Show me! In HIV RT-1, solvent-accessible areas are buried by the dsDNA. Areas covered: 74 A2 for the fingers, 607 A2 for the palm and 514 A2 for the thumb. The polymerase active site (discussed in detail here Active Site) is composed of the palm subdomain at the bottom of the DNA binding cleft. Show me! The template strand of the dsDNA is located about 8 A away from the RNase H site (a position that is unfavorable for cleavage).
Both subunits derive from the same polyprotein precursor and are later processed by the viral protease to yield the final active enzyme. p66 is composed of 560 amino acids; p51 has 440 amino acids. Due to similar origins, each corresponding amino acid sequence in each subunit shows a great deal of homology.
HIV RT-1 Subdomains: fingers palm thumb Binding Cleft The binding cleft of the dsDNA template-primer is composed of the finger, palm and the thumb subdomains. Show me! In HIV RT-1, solvent-accessible areas are buried by the dsDNA. Areas covered: 74 A2 for the fingers, 607 A2 for the palm and 514 A2 for the thumb. The polymerase active site (discussed in detail here Active Site) is composed of the palm subdomain at the bottom of the DNA binding cleft. Show me! The template strand of the dsDNA is located about 8 A away from the RNase H site (a position that is unfavorable for cleavage).
Binding Cleft The binding cleft of the dsDNA template-primer is composed of the finger, palm and the thumb subdomains. Show me! In HIV RT-1, solvent-accessible areas are buried by the dsDNA. Areas covered: 74 A2 for the fingers, 607 A2 for the palm and 514 A2 for the thumb. The polymerase active site (discussed in detail here Active Site) is composed of the palm subdomain at the bottom of the DNA binding cleft. Show me! The template strand of the dsDNA is located about 8 A away from the RNase H site (a position that is unfavorable for cleavage).
In HIV RT-1, solvent-accessible areas are buried by the dsDNA. Areas covered: 74 A2 for the fingers, 607 A2 for the palm and 514 A2 for the thumb.
The polymerase active site (discussed in detail here Active Site) is composed of the palm subdomain at the bottom of the DNA binding cleft. Show me! The template strand of the dsDNA is located about 8 A away from the RNase H site (a position that is unfavorable for cleavage).
The template strand of the dsDNA is located about 8 A away from the RNase H site (a position that is unfavorable for cleavage).