Weis Laboratory Website
Robert M. Weis, Department of Chemistry, University of Massachusetts, Amherst, MA 01003-9336
Physical & Biological Chemistry
Properties and processes of cell membrane and model membrane systems.
Publications (Reverse Chronological)
49. Richter LV, SJ Sandler, RM Weis. 2012. Two isoforms of the Geobacter sulfurreducens PilA have distinct roles in pilus biogenesis, cytochrome localization, extracellular electron transfer and biofilm formation. J Bacteriol (in press)
48. Hutchison JB, RM Weis, AD Dinsmore. 2012. Change of line tension in phase-separated vesicles upon protein binding. Langmuir 28, 5176–5181.
47. Fowler DJ, RM Weis, LK Thompson. 2010. Active signaling complexes of bacterial chemoreceptors do not contain proposed receptor-receptor contacts observed in crystal structures. Biochemistry 49, 1425-1434.
46. Li, Z, KA Stieglitz KA, AL Shrout, Y Wei, RM Weis, B Stec, MF Roberts. 2009. Mobile loop mutations in an archaeal inositol monophosphatase: Modulating three-metal ion assisted catalysis and lithium inhibition. Protein Science 19, 309-318.
45. Tran, HT, J Krushkal, FM Antommattei, DR Lovley and RM Weis. 2008. Comparative genomics of Geobacter chemotaxis genes reveals diverse signaling function. BMC Genomics 9, 471.
44. Esposito, EA, AL Shrout and RM Weis. 2008. Template-directed self-assembly enhances RTK catalytic domain function. J. Biomol. Screen. 13, 810-816.
43. Besschetnova, TY, DJ Montefusco, AE Asinas, AL Shrout, FM Antommattei and RM Weis. 2008. Receptor density balances signal stimulation and attenuation in membrane assembled complexes of bacterial chemotaxis signaling proteins. Proc. Natl. Acad. Sci. USA 105, 12289-12294.
42. Shrout, AL, EA Esposito and RM Weis. 2008. Template-directed assembly of signaling proteins: a novel drug screening and research tool. Chem. Biol. Drug Des. 71, 278-281.
41. Montefusco, DJ, AE Asinas and RM Weis. 2007. Liposome-mediated assembly of receptor signaling complexes. Meth. Enzymol. 423, 267-298.
40. Zhang, P, RM Weis, PJ Peters and S Subramaniam. 2007. Electron tomography of bacterial chemotaxis receptor assemblies. Meth. Cell Biol. 79, 371-382.
39. Montefusco, DJ, AL Shrout, TY Besschetnova and RM Weis. 2007. Formation and activity of template-assembled receptor signaling complexes. Langmuir 23, 3280-3289.
38. Asinas, AE and RM Weis. 2006. Competitive and cooperative interactions in receptor signaling complexes. J. Biol. Chem. 281, 30512-30523.
37. Antommattei, FM and RM Weis. 2006. Reversible methylation of glutamate residues in the receptor proteins of bacterial sensory systems. in "The Enzymes", vol. 24, (S. G. Clarke and F. Tamanoi, eds.) Elsevier, San Diego, pp. 325-379.
36. Weis, RM. 2006. Inch by inch, row by row. Nat. Struct. Mol. Biol. 13, 382-384.
35. Chalah, A and RM Weis. 2005. Site-specific and synergistic stimulation of methylation on the bacterial chemotaxis receptor Tsr by serine and CheW. BMC Microbiology 5, 12.
34. Antommattei, FM, JB Munzner and RM Weis. 2004. Ligand-specific activation of Escherichia coli chemoreceptor transmethylation. J. Bacteriol. 186, 7556-7563.
33. Lefman, J, P Zhang, T Hirai, RM Weis, J Juliani, D Bliss, M Kessel, E Bos, P Peters and S Subramaniam. 2004. Three-dimensional electron microscopic imaging of membrane invaginations in Escherichia coli overproducing the chemotaxis receptor Tsr. J. Bacteriol. 186, 5052-5061.
32. Kott, L, EH Braswell, AL Shrout and RM Weis. 2004. Distributed subunit interactions in CheA contribute to dimer stability; a sedimentation equilibrium study. Biochim. Biophys. Acta 1696, 131-140.
31. Shrout, AL, DJ Montefusco and RM Weis. 2003. Template-directed assembly of receptor signaling complexes. Biochemistry 42, 13379-13385.
30. Weis, RM, T Hirai, A Chalah, M Kessel, P Peters and S Subramaniam. 2003. Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr. J. Bacteriol. 185, 28-35.
29. Yi, X and RM Weis. 2002. The receptor docking segment and s-adenosyl-L-homocysteine bind independently to the methyltransferase of bacterial chemotaxis. Biochim. Biophys. Acta. 1596, 28-35.
28. Murphy, OJ, X Yi, RM Weis and LK Thompson. 2001. Hydrogen exchange reveals a stable and expandable core within the aspartate receptor cytoplasmic domain. J. Biol. Chem. 276, 43262-43269.
27. Li, G and RM Weis. 2000. Covalent modification regulates ligand binding to receptor complexes in the chemosensory system of Escherichia coli. Cell 100, 357-365.
26. Rao, J, J Lahiri, RM Weis and GM Whitesides. 2000. Design, synthesis, and characterization of a high-affinity trivalent system derived from vancomycin and L-lys-D-ala-D-ala. J Amer. Chem. Soc. 122, 2698-2710.
25. Rao, J, L Yan, J Lahiri, GM Whitesides, RM Weis and HS Warren. 1999. Binding of a dimeric derivative of vancomycin to L-lys-D-ala-D-ala-lactate in solution and at a surface. Chem. Biol. 6, 353-359.
24. Rao, J, G Lahiri, L Isaacs, RM Weis and GM Whitesides. 1998. A trivalent system from vancomycin·D-ala-D-ala with higher affinity than avidin.biotin. Science 280, 708-711.
23. Li, J, G Li and RM Weis. 1997. The serine chemoreceptor from Escherichia coli is methylated through an interdimer process. Biochemistry 36, 11851-11857.
22. Seeley, SB, G Wittrock, LK Thompson and RM Weis. 1996. Oligomers of the cytoplasmic fragment from the Escherichia coli aspartate receptor dissociate through an unfolded transition state. Biochemistry 35, 16336-16345.
21. Seeley, SB, RM Weis and LK Thompson. 1996. The cytoplasmic fragment of the Escherichia coli aspartate receptor displays globally dynamic behavior. Biochemistry 35, 5199-5206.
20. Wu, J, J Li, G Li, DG Long and RM Weis. 1996. The receptor binding site for the methyltransferase of bacterial chemotaxis is distinct from the sites of methylation. Biochemistry 35, 4984-4993.
19. Li, J and RM Weis. 1996. Measurements of protein - protein interaction by isothermal titration calorimetry with applications to the bacterial chemotaxis system in "Techniques in Protein Chemistry", vol. 7, (D. Marshak, ed.) pp. 33-44.
18. Li, J, RV Swanson, MI Simon and RM Weis. 1995. The response regulators CheY and CheB exhibit competitive binding to the kinase CheA. Biochemistry 34, 3056-3065.
17. Wu, J, DG Long and RM Weis. 1995. Reversible dissociation and unfolding of the Escherichia coli aspartate receptor cytoplasmic fragment. Biochemistry 34, 3056-3065.
16. Lin, LN, J Li, JF Brandts and RM Weis. 1994. The serine receptor of bacterial chemotaxis exhibits half-site saturation for serine binding. Biochemistry 33, 6564-6570.
15. Long, DG and RM Weis. 1992. Oligomerization of the cytoplasmic from the aspartate receptor of Escherichia coli. Biochemistry 31, 9904-9911.
14. Long, DG and RM Weis. 1992. Escherichia coli aspartate receptor. Oligomerization of the cytoplasmic fragment. Biophys. J. 62, 69-71.
13. Weis, RM. 1991. Fluorescence microscopy of phospholipid monolayer phase transitions. Chem. Phys. Lipids 57, 227-239.
12. Weis, RM, S Chasalow and DE Koshland Jr. 1990. The role of methylation in chemotaxis. Explanation of outstanding anomalies. J. Biol. Chem. 265, 6817-6826.
11. Weis, RM and DE Koshland Jr. 1990. Chemotaxis in Escherichia coli proceeds efficiently from different initial frequencies. J. Bacteriol. 172, 1099-1105.
10. Koshland, DE Jr, DA Sanders and RM Weis. 1988. Roles of methylation and phosphorylation in the bacterial sensing system. Cold Spring Harb. Symp. Quant. Biol. 53, 11-17.
9. Weis, RM and DE Koshland Jr. 1988. Reversible receptor methylation is essential for normal chemotaxis of Escherichia coli in gradients of aspartic acid. Proc. Natl. Acad. Sci. USA 85, 83-87.
8. McConnell, HM, TH Watts, RM Weis and AA Brian. 1986. Supported planar membranes in studies of cell-cell recognition in the immune system. Biochim. Biophys. Acta 864, 95-106.
7. Weis, RM and HM McConnell. 1985. Cholesterol stabilizes the crystal-fluid interface in phospholipid monolayers. J. Phys. Chem. 89, 4453-4459.
6. Weis, RM and HM McConnell. 1984. Two-dimensional chiral crystals of phospholipid. Nature. 310, 49-51.
5. McConnell, HM, LK Tamm and RM Weis. 1984. Periodic structures in monolayer phase transitions. Proc. Natl. Acad. Sci. USA 84, 3249-3253.
4. Weis, RM and HM McConnell. 1982. Molecular basis of cell-cell recognition in CELL FUNCTION AND DIFFERENTIATION: Part A, Volume 102A of Progress in Clinical and Biological Research, G. Akoyunoglou et al. editors. Alan R. Liss, Inc. New York. 331-336.
3. Weis, RM, K Balakrishnan, BA Smith and HM McConnell. 1982. Stimulation of fluorescence in a small contact region between rat basophil leukemia cells and planar lipid membrane targets by coherent evanescent radiation. J. Biol. Chem. 257, 6440-6445.
2. Lin, KC, RM Weis and HM McConnell. 1982. Induction of helical liposomes by Ca2+-mediated intermembrane binding. Nature. 296, 164-165.
1. Smith, LM, RM Weis and HM McConnell. 1981. Measurement of rotational motion in membranes using fluorescence recovery after photobleaching. Biophys. J. 36, 79-91.
Patent
Weis, RM, AL Shrout and DJ Montefusco. 2010. Template-directed assembly of receptor signaling complexes. US 7,678,540 B2.