UMass Amherst Department of Chemistry UMass Amherst

Publications

  1. Fahie, M. A.; Yang, B.; Mullis, M.; Holden, M. A.; Chen, M., Selective detection of protein homologues in serum using an OmpG nanopore. Analytical Chemistry 2015, accepted.
  2. Lein, M.; deRonde, B. M.; Sgolastra, F.; Tew, G. N.; Holden, M. A. Protein transport across membranes: comparison between lysine and guanidinium-rich carriers. BBA Biomembranes 2015, 2980 - 2984. PDF.
  3. Leptihn, S.; Castell, O. K.; Cronin, B.; Lee, E.-H.; Gross, L. C. M.; Marshall, D. P.; Thompson, J. R.; Holden, M.; Wallace, M. I. Constructing droplet interface bilayers from the contact of aqueous droplets in oil. Nat Protoc 2013, 8, 1048–1057. PDF
  4. Lein, M.; Huang, J.; Holden, M. A. Robust reagent addition and perfusion strategies for droplet-interface bilayers. Lab Chip 2013, 13, 2749–2753. PDF
  5. Fischer, A.; Holden, M. A.; Pentelute, B. L.; Collier, R. J. Ultrasensitive detection of protein translocated through toxin pores in droplet-interface bilayers. Proc. Natl. Acad. Sci. U.S.A. 2011, 108, 16577–16581.PDF
  6. Huang, J.; Lein, M.; Gunderson, C.; Holden, M. A. Direct quantitation of peptide-mediated protein transport across a droplet-interface bilayer. J. Am. Chem. Soc. 2011, 133, 15818–15821. PDF
  7. Maglia, G.; Heron, A. J.; Hwang, W. L.; Holden, M. A.; Mikhailova, E.; Li, Q.; Cheley, S.; Bayley, H. Droplet networks with incorporated protein diodes show collective properties. Nat Nanotechnol 2009, 4, 437–440. PDF
  8. Syeda, R.; Holden, M. A.; Hwang, W. L.; Bayley, H. Screening blockers against a potassium channel with a droplet interface bilayer array. J. Am. Chem. Soc. 2008, 130, 15543–15548. PDF
  9. Hwang, W. L.; Chen, M.; Cronin, B.; Holden, M. A.; Bayley, H. Asymmetric droplet interface bilayers. J. Am. Chem. Soc. 2008, 130, 5878–5879. PDF, Supporting Info
  10. Hwang, W. L.; Holden, M. A.; White, S.; Bayley, H. Electrical behavior of droplet interface bilayer networks: experimental analysis and modeling. J. Am. Chem. Soc. 2007, 129, 11854–11864. PDF, Supporting Info
  11. *Holden, M. A.; Needham, D.; Bayley, H. Functional bionetworks from nanoliter water droplets. J. Am. Chem. Soc. 2007, 129, 8650–8655. PDF, Supporting Info
    See also: Nature Highlight, Scientific American, Analytical Chemistry - Lab Fab
  12. *Holden, M. A.; Jayasinghe, L.; Daltrop, O.; Mason, A.; Bayley, H. Direct transfer of membrane proteins from bacteria to planar bilayers for rapid screening by single-channel recording. Nat. Chem. Biol. 2006, 2, 314–318. PDF
    See also: News and Views Nature Chemical Biology PDF
  13. Jung, S. Y.; Holden, M. A.; Cremer, P. S.; Collier, C. P., Two-component membrane lithography via lipid backfilling. ChemPhysChem 2005, 6, 423-426. PDF
  14. Holden, M. A.; Cremer, P. S. Microfluidic tools for studying the specific binding, adsorption, and displacement of proteins at interfaces. Annu Rev Phys Chem 2005, 56, 369–387.PDF
  15. *Holden, M. A.; Bayley, H. Direct introduction of single protein channels and pores into lipid bilayers. J. Am. Chem. Soc. 2005, 127, 6502–6503. PDF, Supporting Info
  16. Kataoka, S.; Gurau, M. C.; Albertorio, F.; Holden, M. A.; Lim, S. M.; Yang, R. D.; Cremer, P. S. Investigation of water structure at the TiO2/aqueous interface. Langmuir 2004, 20, 1662-1666. PDF
  17. Holden, M. A.; Jung, S. Y.; Yang, T. L.; Castellana, E. T.; Cremer, P. S. Creating fluid and air-stable solid supported lipid bilayers. J. Am. Chem. Soc. 2004, 126, 6512-6513. PDF
  18. Holden, M. A.; Jung, S. Y.; Cremer, P. S. Patterning enzymes inside microfluidic channels via photoattachment chemistry. Anal. Chem. 2004, 76, 1838-1843. PDF
  19. Yang, T. L.; Baryshnikova, O. K.; Mao, H. B.; Holden, M. A.; Cremer, P. S. Investigations of bivalent antibodies binding on fluid planar supported phospholipid membranes: The effect of hapten density. J. Am. Chem. Soc. 2003, 125, 4779-4784. PDF
  20. Jung, S. Y.; Lim, S. M.; Albertorio, F.; Kim, G.; Gurau, M. C.; Yang, R. D.; Holden, M. A.; Cremer, P. S. The Vroman effect: A molecular level description of fibrinogen displacement. J. Am. Chem. Soc. 2003, 125, 12782-12786. PDF
  21. Holden, M. A.; Kumar, S.; Castellana, E. T.; Beskok, A.; Cremer, P. S. Generating fixed concentration arrays in a microfluidic device. Sensors and Actuators B 2003, 92, 199-207. PDF
  22. Holden, M. A.; Kumar, S.; Beskok, A.; Cremer, P. S. Microfluidic diffusion diluter: bulging of PDMS microchannels under pressure-driven flow. J. Micromech. Microeng. 2003, 13, 412-418. PDF
  23. Holden, M. A.; Cremer, P. S. Light activated patterning of dye-labeled molecules on surfaces. J. Am. Chem. Soc. 2003, 125, 8074-8075. PDF
  24. Mao, H. B.; Holden, M. A.; You, M.; Cremer, P. S. Reusable platforms for high-throughput on-chip temperature gradient assays. Anal. Chem. 2002, 74, 5071-5075. PDF

Holden Research Group
Department of Chemistry in the College of Natural Sciences
University of Massachusetts Amherst

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